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. 2019 Sep 29;17(1):98–111. doi: 10.1080/15476286.2019.1669405

Figure 1.

Figure 1.

Isolation and characterization of primary bone marrow stromal cells (BMSCs). (A) BMSCs were isolated from BMMNCs which were plated in culture flasks. BMSCs have the tendency to adhere, hence, attached to the surface and proliferate with regular change of media. On day 6, some adhered cells were observed which grown further and on day 13–15, these cells attained optimum confluency (70–80%), hence, mentioned as passage 0. Scale bar represents 100 μm; (B) Flow cytometry characterization of BMSCs at passage 2 showing dual positivity for stromal cells marker (CD90 and CD105) while dual negativity for haematopoietic cell marker (CD34 and CD45); (C) percentage purity of BMSCs showing 96%-99% pure culture. [BMSCs: bone marrow stromal cells; BMMNCs: bone marrow mononuclear cells].