Figure 3.

Silencing MRPS31P5 in human and rhesus fibroblasts as well as silencing H-S in marmoset fibroblasts reduced cell proliferation.

(A) left panel, expression level of the chimeric RNA HNRNPA1L2-SGUT1 in the human fibroblast cell line (HFF), and marmoset fibroblast cell line (CJ-157); right panel, MRPS31P5 is expressed in HFF, while the it does not exist in marmoset. (B) With siRNAs targeting the junction sequence (si-H-S), qRT-PCR showed significantly knocking down of MRPS31P5 in HFF and RF, H-S in CJ-157. (C) With transfection of si-H-S, cell number in HFF, RF and CJ-157 were significantly reduced in compared with si-gl2 group. (d) with siRNAs specific for human MRPS31P5 (si-hMRPS31P5) and rhesus monkey MRPS31P5 (si-rMRPS31P5), qRT-PCR showed significant knocking down of MRPS31P5 in both HFF and RF cells. (e) With transfection of si-hMRPS31P5 and si-rMRPS31P5, the cell numbers in HFF and RF were significantly reduced compared with si-gl2 controls.