Figure 7.

H-S chimeric RNA is a product of cis-SAGe.

(A) The schematic diagram of cis-SAGe assay. RNAs were treated with DNaseI. With a downstream oligo annealing to the exon6 of SUGT1, RNA was reverse transcribed into cDNA. PCR was then performed with a primer pair annealing to the exon5 and intron5 regions of HNRNPA1L2. (B) In bladder cancer cell lines SV-HUC-1 and T24, correct bands were only detected in AMV + group, but not in the group omitting AMV, suggesting the complete removal of DNA template. (C) Same experiment was performed using RNAs extracted from marmoset cell line CJ-157. Same result was observed.