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. 2019 Sep 27;17(1):62–74. doi: 10.1080/15476286.2019.1667214

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 5. — SRRM4 promotes the alternative splicing of TAF1-34ʹ in different non-neuronal cell lines.

RPE1 and U-2 OS derivative cell lines express GFP-SRRM4 as validated by immunoblot (A and C) and immunofluorescence (B and D). The expression of the transgene is verified after 24 h DOX induction. GFP-SRRM4 resides in the nucleus where it co-localizes with the nuclear speckle marker SRSF2 (B and D, lower panels). In both RPE1 and U-2 OS, the induction of SRRM4 results in microexon 34ʹ incorporation into TAF1 mRNA. Plasmids containing cTAF1 and TAF1-34ʹ cDNAs were used as controls. The different PSI quantifications are depicted below each lane (E).