Table 3. Primer sequences of genes used for RT-PCR and quantitative real-time PCR.
| Genes | Primer sequences | Product size (bp) | Tm (°C) |
| Primer sequences of genes used for RT-PCR | |||
| VASA | F: GCAGAAGGAGGAGAAAGTAGTG R: CTCGTCCTGCAAGTATGATAGG |
289 | 56 |
| GPR125 | F: GCGTCATTACGGTCTTTGGAA R: ACGGCAATTCAAGCGGAGG |
199 | 60 |
| GFRA1 | F: CGGGTGGTCCCATTCATATC R: TGGCTGGCAGTTGGTAAA |
411 | 60 |
| THY1 | F: ATCGCTCTCCTGCTAACAGTC R: CTCGTACTGGATGGGTGAACT |
135 | 52 |
| PLZF | F: CGGTTCCTGGATAGTTTGC R: GGGTGGTCGCCTGTATGT |
317 | 54 |
| RET | F: CTCGTTCATCGGGACTTG R: ACCCTGGCTCCTCTTCAC |
126 | 56 |
| UCHL1 | F: AGCTGAAGGGACAAGAAGTTAG R: TTGTCATCTACCCGACATTGG |
265 | 60 |
| FOXP3 | F: CCTCCACAACATGGACTACTT R: TCAGGGGCCAGGTGTAGGGT |
325 | 54 |
| ACTB | F: CATGTACGTTGCTATCCAGGC R:CTCCTTAATGTCACGCACGAT |
250 | 58 |
| Primer sequences of genes used for quantitative real-time PCR | |||
| FOXP3 | F: CTTCAAGTTCCACAACATGCGACC R: TAGATCTCATTGAGTGTCCGCTGC |
102 | 59 |
| ACTB | F: CACTCTTCCAGCCTTCCTTC R: GTACAGGTCTTTGCGGATGT |
104 | 60 |