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. 2019 Dec 20;11(24):12328–12344. doi: 10.18632/aging.102575

Figure 4.

Figure 4

S100A4/p53 axis was the downstream target of METTL1 and miR-149-3p. Real-Time qPCR was conducted to detect the mRNA levels of (A) S100A4 and (B) p53 in CR-CC cells. Western Blot was performed to determine the expression levels of METTL1, S100A4 and p53 in (C, D) CR-HCT116 cells, (E, F) CR-SW480 cells and (G, H) CR-SW620 cells. (I) The binding sites of miR-149-3p and the 3′UTR regions of S100A4 were predicted by online starBase software. Western Blot was used to detect the expression levels of METTL1, S100A4 and p53 in (L, M) CR-HCT116 cells, (N, O) CR-SW480 cells and (P, Q) CR-SW620 cells. (“OE-METTL1” represented “Overexpressed METTL1 group”, and “OE-METTL1+Inhi” represented “Overexpressed METTL1 plus miR-149-3p inhibitor group”). All the experiments repeated at least 3 times. “*” means p < 0.05, “**” means p < 0.01 and “NS” means no statistical significance.