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. 2019 Dec 20;11(24):12328–12344. doi: 10.18632/aging.102575

Table 1. Primer sequences for Real-Time qPCR.

Gene Primer sequences (strand)
β-actin Forward: 5′-CTCCATCCTGGCCTCGCTGT-3′
Reverse: 5′-GCTGCTACCTTCACCGTTCC-3′
U6 Forward: 5′-GACTATCATATGCTTACCGT-3′
Reverse: 5′-GGGCAGGAAGAGGGCCTAT-3′
miR-149-3p Forward: 5′-GGCTCTGGCTCCGTGTCTT-3′
Reverse: 5′-CAGTGCAGGGTCCGAGGTATT-3′
METTL1 Forward: 5′-GAACATCGCCTGTCTCCGAA-3′
Reverse: 5′-TCGCTTAAAGTGTGGGTCCG-3′
S100A4 Forward: 5′-GATGTGATGGTGTCCACCTT-3′
Reverse: 5′-ATTTCTTCCTGGGCTGCTTA-3′
p53 Forward: 5′-CAGCCAAGTCTGTGACTTGCACGTAC-3′
Reverse: 5′-CTATGTCGAAAAGTGTTTCTGTCATC-3′

The genes included β-actin, U6, miR-149-3p, METTL1, S100A4 and p53.