Table 1. Primer sequences for Real-Time qPCR.
Gene | Primer sequences (strand) |
β-actin | Forward: 5′-CTCCATCCTGGCCTCGCTGT-3′ Reverse: 5′-GCTGCTACCTTCACCGTTCC-3′ |
U6 | Forward: 5′-GACTATCATATGCTTACCGT-3′ Reverse: 5′-GGGCAGGAAGAGGGCCTAT-3′ |
miR-149-3p | Forward: 5′-GGCTCTGGCTCCGTGTCTT-3′ Reverse: 5′-CAGTGCAGGGTCCGAGGTATT-3′ |
METTL1 | Forward: 5′-GAACATCGCCTGTCTCCGAA-3′ Reverse: 5′-TCGCTTAAAGTGTGGGTCCG-3′ |
S100A4 | Forward: 5′-GATGTGATGGTGTCCACCTT-3′ Reverse: 5′-ATTTCTTCCTGGGCTGCTTA-3′ |
p53 | Forward: 5′-CAGCCAAGTCTGTGACTTGCACGTAC-3′ Reverse: 5′-CTATGTCGAAAAGTGTTTCTGTCATC-3′ |
The genes included β-actin, U6, miR-149-3p, METTL1, S100A4 and p53.