Figure 3.

Klotho binds to Wnt and blocks Wnt-mediated gene transcription. In cartilage tissue, the binding of Dvl to Frizzled, Klotho to Wnt1, Wnt4, Wnt7a and β-catenin to MMP2, 9 promoters were detected. (A, B) The Co-IP method was used to detect the binding of Dvl to Frizzled, and Klotho to Wnt1, Wnt4, Wnt7a in cartilage tissue after operation, (C) and quantitative data for Dvl to Frizzled, and Klotho to Wnt1, Wnt4, Wnt7a are presented. (D, E) The binding of β-catenin to MMP2, 9 promoters was detected by ChIP method. Data are the mean ±SD, n=8 mice.. *P < 0.05, **P < 0.01.