Figure 4.

C13 inhibited OGD-R-induced oxidative stress in neuronal cells. SH-SY5Y human neuronal cells (A–D) or the primary murine hippocampal neurons (E and F) were pretreated with C13 (10 μM) for 2h, followed by OGD-R stimulation for applied time, ROS contents were tested by the appropriate assays (A, B and E); Mitochondrial depolarization and lipid peroxidation levels were tested by JC-1 dye assay (C and F) and TBAR activity assay (D), respectively. Bars stands for mean ± standard deviation (SD, n=5). * p<0.05 vs. “Mock” cells. ^# p<0.05 vs. cells with “OGD-R” treatment (no C13 pretreatment). Each experiment was repeated three times with similar results obtained.