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. Author manuscript; available in PMC: 2020 Jan 8.
Published in final edited form as: Biochem J. 2015 Feb 1;465(3):479–488. doi: 10.1042/BJ20140582

Figure 6. Effects of POR deletion in liver or liver and gut on blood concentrations of nelfinavir, paroxetine and midazolam.

Figure 6

WT and ERL mice (n = 3) were pre-treated with corn oil or 3MC (4 or 40 mg/kg), as described in the Experimental section. A single dose of nelfinavir (50 mg/kg p.o.), paroxetine (10 mg/kg p.o.) or midazolam (5 mg/kg p.o.) was administered on day 14 after 3MC treatment and a pharmacokinetic protocol carried out as described: (A) nelfinavir; (B) paroxetine; (C) midazolam. Black square, WT + Corn oil; black circle, ERL + 4 mg/kg 3MC (liver POR deletion); grey square, ERL + 40 mg/kg 3MC (liver and gut POR deletion). Pharmacokinetic analysis was conducted as described, data shown are means ± S.E.M. Inset: liver microsomal fractions were prepared and immunoblotted for POR and GRP78 (loading control) as described in the Experimental section.