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. 2020 Jan 1;16(2):330–341. doi: 10.7150/ijbs.37781

Figure 4.

Figure 4

Effects of G721-0282 on the STAT3 pathway. (A) The cells were treated with the indicated concentration (10, 30, and 50 μM) of G721-0282 for 6 h. The equal amounts of lysates were analyzed by Western blotting and detected with antibodies against phospho-Src (p-Src), Src, phospho-STAT3 (p-STAT3), and STAT3. (B) 6 h after 50 μM G721-0282 treatment, the cells were fixed and permeabilized. p-STAT3 (green) was immunostained with rabbit anti-p-STAT3 antibody, followed by Alex-488-conjugated secondary antibody. And then the cells were stained with DAPI (a nuclear marker, blue). The bottom panels show the merged images of the first and second panels. Scale bar: 20 μm. These data were representative of three independent experiments.