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. 2020 Jan 1;16(2):251–263. doi: 10.7150/ijbs.38214

Figure 3.

Figure 3

KO of LRRC75A-AS1 enhanced TJ of MAC-T cells. (A) Gene expressions of Claudin-1, Occludin and ZO-1 were analyzed by RT-qPCR. (B) Claudin-1, Occludin and ZO-1 expression in KO-Control and KO-LRRC75A-AS1 cells were analyzed by western blot. GAPDH was used as an internal control. (C) The relative quantitative analysis of western blot result was performed. *P<0.05, **P<0.01. (D) Immunofluorescence of Claudin-1, Occludin and ZO-1 was observed under laser confocal microscope. Nuclei were stained with DAPI. Scale bar: 50 µm. (E) The semiquantitative analysis of immunofluorescence was performed by using Image J.