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KO of LRRC75A-AS1 attenuated the activation of inflammation. (A) Inflammatory factors were detected by RT-qPCR. (B) Western blot on nuclear NF-κB subunit p65 and total phosphorylated p65 was performed to detect the activation of NF-κB pathway. (C) Relative quantitative analysis was performed. H3 was used as control in nucleus. GAPDH was regarded as control in cytoplasm. (D) The activation of NF-κB pathway was tested by dual luciferase reporter gene assay.
