Figure 6.

Ruxolitinib treatment reduced renal tubular cell apoptosis and oxidative stress in UUO kidneys. (A-B) Apoptotic cells in kidney tissue sections were detected by TUNEL staining, and counted. Scalebar, 50 µm. (C-D) Expression of cleaved caspase-3 in kidney tissue sections was detected by Immunohistochemistry, and the positive cells were counted. Scalebar, 50 µm. (E-F) Expression of cleaved caspase-3 in kidney tissue lysates was detected by Western blot, and quantified by densitometry. (G) Relative levels of T-SOD and MDA in kidney tissues were detected by corresponding kits. Mean ± SEM, n=5. *p<0.05, ***p<0.001. RUX: Ruxolitinib; Cl-caspase-3: Cleaved caspase-3.