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25-HC suppresses myocardial apoptosis in IR hearts. (A) Representative images of TUNEL staining showing cardiac cell apoptosis. (B) The percentage of apoptotic cells was shown. (C) The protein levels of T-caspase-3, Bax, Bcl-2, and PARP1 in left ventricular tissues. (D) Statistical results were shown. (E) The mRNA levels of TNF-α, IL-6, and IL-1β in left ventricular tissues. Mean ± SEM. N = 5-6 per group, *P < 0.05 vs. vehicle group.
