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The PARP activity was inhibited by 25-HC in vitro and in vivo. (A) Nuclear extracts from primary mice myocardiocytes were incubated with different concentrations of 25-HC and then subjected to Western blot assay with anti-PAR antibody. (B) Neonatal C57BL/6 murine ventricular myocytes were subjected to H2O2 (300 μmol/L) stimulation to measure protein level of PAR.
