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. 2020 Jan 1;16(2):216–227. doi: 10.7150/ijbs.38722

Figure 5.

Figure 5

sCD83 increases IRF1 expression in sTh2 cells. sTh2 cells were prepared from blood samples collected from 6 AR patients. The sTh2 cells were treated with the procedures denoted on the x axis of panel A. (A) Bars indicate IRF1 mRNA levels in sTh2 cells. CD154d: CD154-deficient sTh2 cells (prepared by CRISPR). Control: sTh2 cells were treated with control CRISPR reagents. Data of bars are presented as mean ± SEM. Each dot in bar present data obtained from one independent experiment. Statistics: ANOVA or t test. (B) Proteins extracted from the 6 sTh2 cell samples were pooled and analyzed by Western blotting (repeated 3 times); immunoblots show IRF1 proteins in sTh2 cells (representing 3 independent experiments). (C) Immunoblot image analysis data of panel B.