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. 2020 Jan 1;16(2):272–283. doi: 10.7150/ijbs.37906

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Tumor necrosis factor-α induced protein 8 (TIPE) and vascular endothelial growth factor receptor 2 (VEGFR2) are highly expressed in CRC and act as oncogenes. (a) TIPE expression in five pairs of fresh human CRC tumor tissues and matched adjacent tissues analyzed by immunohistochemistry staining. (b) Representative results of TIPE expression in five pairs of fresh CRC tumor tissues and adjacent tissues as detected by Western blot; T indicates tumor tissue, and N indicates normal tissue. (c) Comparison of TIPE mRNA expression levels in human CRC tissues and matched adjacent tissues. TIPE mRNA expression was quantified by qRT-PCR and normalized to that in the matched adjacent normal tissues. (d) Through immunohistochemical staining, VEGFR2 expression was analyzed in five randomly selected pairs of CRC tumor tissues and matched adjacent tissues. (e) Comparison of VEGFR2 mRNA expression levels in human CRC tissues and matched adjacent tissues. VEGFR2 mRNA expression was quantified by qRT-PCR and normalized to that in the matched adjacent normal tissues.