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. 2020 Jan 1;16(2):272–283. doi: 10.7150/ijbs.37906

Figure 2.

Figure 2

Knockdown of TIPE downregulates VEGFR2 expression and inhibits cell proliferation, cell migration and angiogenesis. (a) qRT-PCR of TIPE expression in HCT116 cells infected with lentiviral shTIPE or control shRNA. TIPE mRNA expression was quantified by qRT-PCR and normalized to that in the shRNA control cells. (b) TIPE expression in HCT116 cells infected with lentiviral shTIPE or control shRNA according to Western blot analysis. (c) Expression of VEGFR2 in shTIPE and shRNA control HCT116 cells based on qRT-PCR assays. (d) Tumor cell culture conditioned medium (TCM) from HCT116 cells stably transduced with shTIPE or control shRNA inhibited the proliferation of HUVECs as determined by CCK-8 assays. (e) Representative immunofluorescence (IF) images demonstrated that the level of TIPE has an effect on the expression of microfilaments and initial pseudopod extension in the stably transduced HCT116 cell lines. Scale bar, 50 µm. (f) Representative images (left panel) and quantification (right panel) of tube formation of HUVECs treated with TCM derived from control HCT116 cells or HCT116 cells with stable TIPE knockdown. The data were collected from four independent experiments using different batches of cells. Scale bar, 500 μm. **p <0.01, ***p<0.001.