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. 2019 Sep 2;39(2):368–384. doi: 10.1038/s41388-019-0993-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — β3-AR is constitutively expressed in murine and human neuroblastoma cells and it is involved in cell survival. a WB and relative densitometric quantification analysis, showing expression of β3-AR protein in murine (Neuro-2A) and human (BE(2)C, SK-N-BE(2)) NB cell lines in normoxic (N) and hypoxic condition (H). Results were normalized to the expression of β-actin and reported as mean ± SD, fold change over controls (normoxic condition), set as 1. Blots are representative of three independent experiments. Significance was calculated by Unpaired t-test analysis with equal SD (*P < 0.05). b Immunofluorescence staining of β3-AR on tumor sections of two NBs (NB1, NB2) derived from human patients, showing numerous β3-AR positive cells. Images are representative of similar results obtained for n = 6. c MTT survival assay in Neuro-2A, BE(2)C and SK-N-BE(2) NB cell lines, treated with different concentration of SR59230A for 24 h. Results are reported as mean ± SD of three independent experiments performed in triplicate. Significance was calculated by one-way ANOVA analysis followed by Bonferroni’s post hoc test (ns = not significant, *P < 0.05, **P < 0.01, ****P < 0.0001)