Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 2;39(2):368–384. doi: 10.1038/s41388-019-0993-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — β3-AR blockade modulates S1P signaling in human neuroblastoma BE(2)C cells. a RT-PCR of human NB BE(2)C cell line treated with 1 μM SR59230A for 24 h. Change of mRNA expression levels of receptors (S1P₁, S1P₂, S1P₃), metabolic enzymes (SK1, SK2, SPL) and transporter (Spns2) of S1P are reported as mean ± SD of three independent experiments performed in triplicate, using the 2^(-ΔΔCt) method as described in Methods section. Data were normalized to β-actin RNA expression and values of treated samples reported as fold change over control, set as 1. Significance was calculated by Unpaired t-test analysis with equal SD (**P < 0.01). b WB and relative densitometric quantification analysis, showing protein expression levels of SK1, SK2, and S1P₂ in NB BE(2)C cell line after 24 h of 1 μM SR59230A treatment. Results were normalized to the expression of β-actin and reported as mean ± SD, fold change over control, set as 1. Blots are representative of three independent experiments. Significance was calculated by Unpaired t-test analysis with equal SD (*P < 0.05, ***P < 0.001). c WB and relative densitometric quantification analysis, showing protein expression levels of β2-AR, β3-AR, SK2 and S1P₂ in NB BE(2)C cell line after molecular silencing of β2- and β3-AR. Results were normalized to the expression of β-actin and reported as mean ± SD, fold change over control, set as 1. Blots are representative of three independent experiments. Significance was calculated by one-way ANOVA analysis followed by Bonferroni’s post hoc test (ns = not significant, *P < 0.05, **P < 0.01, ****P < 0.0001)