Figure 5.

Gene-corrected CS-NSCs show increased NER ability and decreased susceptibility to UV-induced apoptosis. (A) Immunostaining of the NSC markers Nestin, PAX6, and SOX2 in the CS-NSCs and GC-NSCs. The nuclei were stained with Hoechst 33342. Scale bar, 50 μm. ERCC6^mut represents CS-NSCs, ERCC6^GC represents GC-NSCs. (B) Western blot analysis showing increased protein levels of ERCC6 in GC-NSCs. β-Actin was used as the loading control. (C) CPD immunostaining in CS-NSCs and GC-NSCs in the absence or presence of 5 J/m² UV exposure. Nuclei were stained with Hoechst 33342. Scale bar, 50 μm. Over 300 nuclei were used for calculations. The data are shown as the mean ± SEM, ***P < 0.001. a.u., arbitrary units. (D) Apoptosis analysis of CS-NSCs and GC-NSCs at 48 h after 5 J/m² UV irradiation. Quantitative data are presented as the mean ± SEM, n = 3, *P < 0.05, ***P < 0.001. (E) Western blots showing PARP cleavage in CS-NSCs and GC-NSCs in the absence or presence of 5 J/m² UV exposure. GAPDH was used as a loading control. Quantitative data are presented as the mean ± SD, n = 3, *P < 0.05, ns, not significant