Figure 8.
Gene-corrected CS-MSCs generated under a cGMP-compliant condition displayed alleviated aging defects and decreased susceptibility to UV-induced apoptosis. (A) Clonal expansion assay showing the cell proliferation ability of CS-MSCs and GC-MSCs. The cells were stained with crystal violet after a two-week culture, and the relative intensity of the crystal violet was quantified. Data are presented as the mean ± SEM, n = 4, **P < 0.01. Scale bar, 50 μm. (B) SA-β-Gal staining of CS-MSCs and GC-MSCs. The percentages of SA-β-Gal-positive cells are shown in the right panel. Data are presented as the mean ± SEM, n = 3, **P < 0.01. Scale bar, 50 μm. (C) Apoptosis analysis of CS-MSCs and GC-MSCs 48 h after 10 J/m2 UV irradiation. Quantitative data are presented as the mean ± SEM, n = 3, ***P < 0.001. (D) Western blots showing PARP cleavage of CS-MSCs and GC-MSCs in the presence of 10 J/m2 UV exposure. β-Actin was used as a loading control. (E) Fat pad transplantation with CS-MSCs and GC-MSCs. Left: representative immunofluorescent images showing neovascularization; right: the number of hCD31-positive vessels calculated based on 24 slices from inconsecutive frozen sections. Data are presented as the mean ± SD, n = 3 for each group, **P < 0.01. Scale bar, 50 μm