Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 8;10:48. doi: 10.1038/s41598-019-56880-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Impairment of the insulin signalling in the obese mother’s offspring. Hepatic pAKT protein levels were evaluated by Western blot in control and obese dams’ offspring (P28) after a bolus injection of saline or regular insulin (5 UI) through the abdominal cava vein. The expression of the protein was measured after 45 seconds of insulin administration. The percent expression of control (AKT) is shown (means ± SD, n = 6 per group). (a) Hepatic pAKT protein levels were evaluated by Western blot in control and obese dams’ offspring (P28) after an ex vivo experiment where the tissue was treated with insulin (100 nM) for 10 minutes in the cell media. The percent expression of control (GAPDH) is shown (means ± SD, n = 6 pups for SC-O and n = 4 pups for HFD-O). (b) Hepatic pAKT protein levels were evaluated by Western blot in KOα7^Alb-Cre and WT mice after an ex vivo experiment where the tissue was treated with insulin (100 nM) for 10 minutes in the cell media. The percent expression of control (GAPDH) is shown (means ± SD, n = 6 pups for SC-O and n = 4 pups for HFD-O). (c) Statistical significance was analysed by Student’s t-test for analysis of two groups (*p < 0.05).