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. 2020 Jan 8;9(1):4. doi: 10.1038/s41389-019-0188-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a MDA-MB-231 and BT-549 cells were infected with control shRNA and two different SGK196 shRNAs. After antibiotic selection, knockdown of SGK196 was validated by Western blotting analysis. b MDA-MB-231 and BT-549 cells stably expressing HA-SGK196 were validated by western blotting analysis. c–f The resultant stable cell lines with SGK196 knockdown and overexpression were subjected to migration assays (c, d) and invasion assays (e, f). The data are presented as the mean ± S.D, **p < 0.01.