Table 1.
5FC-resistant isolates whole genome sequenced or successfully genotyped by Sanger sequencing.
| Strain Name | Original Genotype | Putative Resistance Allele |
|---|---|---|
| R265-1 | Wildtype | fur1 1134delT |
| R265-2 | Wildtype | ~18.5 kb deletion spanning fur1 |
| R265-3 | Wildtype | fur1 1003delT, mutation detected via Sanger |
| R265-4 | Wildtype | fur1 1136delT, mutation detected via Sanger |
| R265-5 | Wildtype | uxs1 1520delT |
| R265-6 | Wildtype | fur1 1440delA, mutation detected via Sanger |
| R265-7 | Wildtype | ~14.7 kb deletion spanning fur1 |
| R265-8 | Wildtype | ~26.7 kb deletion spanning fur1 |
| RBB17-1 | msh2Δ::NEO | |
| RBB17-2 | msh2Δ::NEO | |
| RBB17-3 | msh2Δ::NEO | |
| RBB17-4 | msh2Δ::NEO | |
| RBB17-5 | msh2Δ::NEO | fur1 1358delA in 6 base homopolymer |
| RBB17-6 | msh2Δ::NEO | |
| RBB17-7 | msh2Δ::NEO | |
| RBB17-8 | msh2Δ::NEO | fur1 G448A (splice acceptor) |
| RBB18-1 | msh2Δ::NEO | |
| RBB18-2 | msh2Δ::NEO | fur1 1358delA in 6 base homopolymer |
| RBB18-3 | msh2Δ::NEO | |
| RBB18-4 | msh2Δ::NEO |
fcy2 Trp167Stop uxs1 Asp306Gly |
| RBB18-5 | msh2Δ::NEO | fur1 1358delA in 6 base homopolymer |
| RBB18-6 | msh2Δ::NEO | fur1 1027delT in 5 base homopolymer |
| RBB18-7 | msh2Δ::NEO | |
| RBB18-8 | msh2Δ::NEO | uxs1 1182insC in 7 base homopolymer |
| RBB18-9 | msh2Δ::NEO | uxs1 Tyr217Cys |