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. 2020 Jan 8;11:105. doi: 10.1038/s41467-019-13756-4

Fig. 2. RNA-LL37 promote the release of cytokines and chemokines.

Fig. 2

Cytometric bead array (a–c) or ELISA (d) for TNF (a), IL-6 (b), IL-1β (c), or MIP-1β (d) secreted from PMNs stimulated for 4 h as indicated (n = 6). Flow cytometric cell counts of migrated CD4+ T cells (e, h), CD8+ T cells (f) and CD14+HLA-DR+ monocytes (g) quantified in transwell assays with total PBMCs in the upper and MIP-1β (30 and 150 pg/ml) (e–g, n = 6–7) or R848, RNA with and without LL37 (h, n = 3–7) in the lower chamber. i ELISA of MIP-1β secreted from psoriasis PMNs (n = 3 patients, 10 healthy controls, squares, chequered bars) or PMNs from sex and age-matched healthy donors. Only responses to treatment compared. j as in I but ELISA for LL37 (n = 4 patients, 7 healthy donors). Panels (a–j) represent combined data (mean + SD) from ‘n’ biological replicates (each dot represents one donor). *p < 0.05 according to Friedmann (a, b, e, f) or Kruskal–Wallis (c, h) test with Dunn’s correction, or one-way ANOVA with Dunnett’s (d, g, h) or Sidak (i) correction or Student’s t-test (j). Source data is provided as a Source data file.