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. 2020 Jan 8;10:47. doi: 10.1038/s41598-019-56842-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Alpha-glucosidase inhibitor candidates’ effect on HBV replication. (A) HBsAg level after treatment with alpha-glucosidase inhibitor candidates. HBsAg levels in the supernatant for corresponding alpha-glucosidase inhibitor candidates on day 22. The vertical axis depicts the dilution level of HBsAg in the medium. (B) The relationship between HBV-DNA and human albumin. The line chart and bar graph show the amount of HBV-DNA (right vertical axis) and the relative amount of HBsAg (left vertical axis), respectively. (C) The relationship between HBV-DNA and the concentration of alpha-glucosidase inhibitor candidates. The vertical and horizontal axis is the ratio of HBV-DNA in non-treated samples and the concentration of alpha-glucosidase inhibitor candidates, respectively. Each IC₅₀ is also described. (D) The level of cccDNA in PXB cells. An asterisk indicates a significant difference (p < 0.05). Each experiment was repeated three times.