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. 2020 Jan 8;10:24. doi: 10.1038/s41598-019-56870-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Expression of CRKL protein in LSCC cell lines and non-tumor squamous epitelium of the larynx – CRKL present in the basal layer (primary objective magnification 20x). (B) CRKL mRNA expression shows a good corelation with observed protein expression. (C) Examples of CRKL protein expression in LSCC cases. IHC staining of CRKL protein in laryngeal squamous cell carcinoma. The intensity of the expression (+++ strong, ++ moderate, + weak). Nuclei counterstained with hematoxylin show nuclear-cytoplasmic expression.