Table 3.

Characteristics of cDNA primer sequences and reaction conditions.

Gene	Primers sequences	Amplicon length (bp)	Annealing Tm (°C)	Efficiency
CRKL	F: 5′ TCAACCTCAGACCACAACTC 3′	166	56	0.91
NM_005207	R: 5′ GATGTCACCAAC—CTCTAATGC 3′
ACTB	F: 5′ CACCACACCTTCTACAATG 3′	162	60	1.00
NM_001101	R: 5′ TAGCACAGCCTGGATAG 3′
GAPDH	F: 5′ GTCGG—AGTCAACGGATT 3′	220	60	0.98
NM_001256799	R: 5′ CCTGGAAGATGGTGATGG 3′

^*The hyphen in the primer sequence denotes the exon/exon boundary.