Figure 4.

RNA Expression Differences between FOXI3 MT and WT. A) Covariance-based Principal Component Analysis (PCA) scatter plot describing the relationships between FOXI3 MT (yellow) and WT (blue) samples using expression for 125 gene probes (Affymetrix Clariom_S_Mouse) identified to be dysregulated between them (Welch-modified T-test P < 0.05 & absolute fold-difference of means ≥ 1.5x). B) Volcano plot summary of the gene probes tested for dysregulated expression between samples representing FOXI3 MT and WT using the Welch-modified T-test. Blue-colored circles indicate gene probes having both a Welch-modified T-test P < 0.05 and fold-difference of means ≥ (+)1.5x between FOXI3 MT and WT samples (n=97). Yellow-colored circles indicate gene probes having both a Welch-modified T-test P < 0.05 and fold-difference of means ≤ (−)1.5x between FOXI3 MT and WT samples (n=28). Gray-colored circles indicate gene probes not observed to have either a Welch-modified T-test P < 0.05 or an absolute fold-difference of means ≥ 1.5x between FOXI3 MT and WT samples (n=19,792). C) Heat map describing the relationship between FOXI3 MT and WT samples by Euclidean-based dendrogram in columns (agglomeration method = complete) using expression for the same 125 gene probes described in A). Relationships across these probes is similarly described by dendrogram in rows. Expression observed per probe is summarized using a two-color coding with 64-levels of blue (low expression) to yellow (high expression).