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. Author manuscript; available in PMC: 2020 Jun 9.
Published in final edited form as: Nature. 2019 Dec 9;577(7789):244–248. doi: 10.1038/s41586-019-1786-y

Extended Data Figure 7: Fusion of EcoRI restriction enzymes to ORF152 enables immune activity.

Extended Data Figure 7:

a, Strain PAO1 was engineered to express EcoRI or EcoRI-ORF152 fusion protein. Plaque assays were conducted as in Fig. 1a and quantified (n=3). b, Growth curves were conducted, monitoring the OD600 of PAO1 cells infected with the indicated ΦKZ multiplicity of infection (MOI). c, Live fluorescence imaging of P. aeruginosa strains engineered to express EcoRI or EcoRI-ORF152. DAPI stain shows the phage DNA. d, Live fluorescence imaging of P. aeruginosa strains engineered to express EcoRI, or EcoRI-ORF152. DAPI stain shows the phage DNA within the shell. Wide field of view. All plaque assays were replicated 3 times with similar results. Growth curve experiments were replicated twice with similar results. Microscopy was replicated twice with similar results.