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. Author manuscript; available in PMC: 2020 Jun 9.
Published in final edited form as: Nature. 2019 Dec 9;577(7789):244–248. doi: 10.1038/s41586-019-1786-y

Figure 2: CRISPR-Cas and restriction proteins are excluded from ΦKZ’s nucleus-like structure.

Figure 2:

a, Fluorescence microscopy of P. aeruginosa immunostained for Cas9, DAPI stain shows the phage DNA within the nucleus-like structure. Live fluorescence microscopy of P. aeruginosa strains engineered to express b, II-A Cas9 or I-C or I-F Cas8 or Cas3 proteins fused to Cherry, c, a Cherry-HsdR fusion, d, Immunostained for Myc-ORF152 (top panels), or live imaging of ORF152 and TopA proteins fused to Cherry, or Cherry alone. All experiments were replicated ≥ 2 times with similar results.