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. Author manuscript; available in PMC: 2021 Jan 7.
Published in final edited form as: Anal Chem. 2019 Dec 23;92(1):1346–1354. doi: 10.1021/acs.analchem.9b04505

Figure 1.

Figure 1.

Identification of m5C-interacting proteins. (A) A schematic overview of the SILAC-based quantitative proteomics method for discovering m5C reader proteins. Shown is the workflow for a forward SILAC labeling experiment. (B) A scatter plot showing the proteins identified in RNA pull-down assay in HeLa cells. Displayed are results based on three forward and three reverse SILAC labeling experiments. (C) Representative ESI-MS for the [M+2H]2+ ions of YTHDF2 peptide SINNYNPK revealing the preferential binding of YTHDF2 toward the m5C probe in both forward (up) and reverse (bottom) SILAC experiments.