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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: J Allergy Clin Immunol. 2019 Oct 15;145(1):312–323. doi: 10.1016/j.jaci.2019.08.034

Figure 2. O3 induced il6 and sftpd mRNA expression in airway epithelial cells but diminished SP-D protein expression in the BAL. LGM2605 alleviated these effects.

Figure 2.

(A) RNA was extracted from bronchial brush biopsies, and expression of the il6 and sfptd genes were measured by qPCR. ΔΔCt (gapdh and rpl32), fold of baseline/day 1; each macaque served as its own control. (B) Representative reducing SDS-page western blot detecting non-human primate BAL SP-D. Mouse (and human; not shown) recombinant SP-D (rSP-D) was used as a loading control (C) Densitometric values (reducing SDS-page gels, ImageJ, (% of day 1; each macaque served as its own control). (D) Schematic depiction of the dual antagonistic effects of O3 and LGM2605. O3 induces IL-6 production in an autocrine manner that increases SP-D synthesis. O3-induced oxidative damage diminishes released SP-D. LGM2605 interacts with these events. Mean±SEM of n=5–6; *p<0.05 (within groups: day 1 vs. day 8; Student’s paired t-test) #p<0.05 (between groups; Two-way ANOVA).