Figure 5. O₃ activated BAL CD4⁺ T cells.

(A-B) CD4⁺ T cells (live CD45⁺CD3⁺CD4⁺CD8⁻ cells) and CD8+ T cells (live CD45⁺CD3⁺CD4⁻CD8⁺ cells) were quantified (cells/mL BAL ×10³). (C-E) Proportion of CD4⁺ and CD8⁺ T cells that were CD25⁺ (mean±SEM in flow plots and bar graphs). (F-G) IFNγ and IL-4 expression (MFI) was measured in CD4⁺ and CD8⁺ T cells (% of day 1; each macaque served as its own control). Mean±SEM of n=5–6; *p<0.05, **p<0.01 (within groups: day 1 vs. day 8, F-G: Student’s paired t-test; A-E: Two-way ANOVA).