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. Author manuscript; available in PMC: 2021 Jan 8.
Published in final edited form as: J Am Chem Soc. 2019 Dec 26;142(1):614–622. doi: 10.1021/jacs.9b12265

Figure 4.

Figure 4.

Simultaneous, two-color voltage and Ca2+ imaging in hippocampal neurons with RhoVR-Halo 15 and GCaMP6s. a) Transmitted light image of neurons expressing cell-surface HaloTag and cytosolic GCaMP6s. b) Merged widefield fluorescence microscopy image depicting 15 (magenta) and GCaMP6s (green) staining. Individual channels of the same neuron show c) membrane-associated 15 and d) cytosolic GCaMP6s. e) Plot of ΔF/F vs time for 15 (magenta) and GCaMP6s (green). f) Expanded time scale of the boxed region in panel (e). Scale bar is 20 μm.