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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: J Allergy Clin Immunol. 2019 Oct 9;145(1):324–334. doi: 10.1016/j.jaci.2019.09.026

Figure 1. WASp is required to maintain the physiological Golgi morphology in T and B cells.

Figure 1.

(A) Confocal immunofluorescence images of the human, primary Th cells triple-labelled with the indicated reagents. Displayed images are the collapsed composites of 20–30 z-stack images acquired at x63 magnification, and are representative of at least 50 cells for each condition. (B) confocal images of Th cells and B cells (WASp+, WASp−) dual-labelled with the indicated reagents showing Golgi morphology under basal, steady-state conditions. (C) Western blot showing the presence or absence of the indicated protein in 4 isogenic Th cell lines lacking either WASp (WKO), GOLPH3 (GKO), both WASp and GOLPH3 (WGKO), or wild-type (WT). (D) Confocal immunofluorescence images of the Golgi morphology in the same 4 Th cell lines shown in panel C and B cells (WT and WAS). (E) Box-and-whisker plots (whiskers@10–90%, horizontal bar denotes median, “+” denotes mean) generated from 3D (z-stack) images of at least n=100 cells from n=3 independent experiments. p**** ≤ 0.0001, Mann-Whitney nonparametric.