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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: J Allergy Clin Immunol. 2019 Oct 9;145(1):324–334. doi: 10.1016/j.jaci.2019.09.026

Figure 5. F-actin depolymerization does not impede DNA damage induced Golgi dispersal response in WASp-sufficient Th cells.

Figure 5.

(A) Confocal immunofluorescence images of the human Th cells (WT, ND1 donor) multi-labelled with the indicated reagents after treating Th cells with Latrunculin A (Lat-A, F-actin depolymerizing agent), Brefeldin A (BFA, inhibitor of protein transport/secretion that induces spontaneous Golgi dispersal), or DMSO control. Note, Lat-A per se does not induce DNA damage (γH2A.X foci) and yet disperses Golgi. Box-and-whisker plots depict the magnitude of GDR under Lat-A or DMSO treatment in WT and WKO Th cells. Other descriptions of the images, plots, and p-values as per Figure 2A. (B) Confocal immunofluorescence images of human Th cells, WT, WKO, GKO from ND1 donor multi-labelled with the indicated reagents after treating Th cells with CPT alone or CPT followed by Lat-A (CL) or DMSO control. Box-and-whisker plots (on right) depict the magnitude of GDR under indicated conditions. *p<0.05, **p<0.001, ****p<0.0001 Mann-Whitney nonparametric.