Extended Data Table 1 |.
Cryo-EM data collection, structure refinement and model statistics.
| HMPV L:P complex (EMDB-20651) (PDB 6U50) | |
|---|---|
| Data collection and processing | |
| Magnification (nominal/calibrated) | 31,000/40,322.58 |
| Voltage (kV) | 300 |
| Electron exposure (e-/Å2) | 48 |
| Defocus range (μm) | 1.5–3.0 |
| Pixel size (Å) | 1.24 |
| Symmetry imposed | Cl |
| Initial particle images (no.) | 5,381,943 |
| Final particle images (no.) | 302,346 |
| Map resolution (Å) | 3.7 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | ∞-3.7 |
| Refinementa | |
| Initial model used (PDB code) | - |
| Model resolution (Å) | 3.7 |
| FSC threshold | 0.5 |
| Model resolution range (Å) | ∞-3.7 |
| Map sharpening B factor (Å2) | −188.25 |
| Model composition | |
| Non-hydrogen atoms | 12,977 |
| Protein residues | 1,623 |
| Ligands | - |
| B factors (Å2) | |
| Protein | 15.62 (5.27–52.43) |
| Ligand | - |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.004 |
| Bond angles (°) | 0.717 |
| Validationb | |
| MolProbity scorec | 1.31 (98th percentile) |
| Clashscore | 2.13 |
| Poor rotamers (%) | 0.00 |
| Ramachandran plot | |
| Favored (%) | 100.00 |
| Allowed (%) | 0.00 |
| Disallowed (%) | 0.00 |
1
Refinement statistics were obtained with program Phenix (phenix.real_space_refinement) (ref 30) except otherwise noted.
2
Molprobity score was obtained from the Molprobity online server (100th percentile is the best among structures within the resolution specified) and the present structure is in the 98th percentile (N=27675, 0 – 99Å)
3
The assignment of Pro-1041 as a cis-Proline is supported by electron density showing a hydrogen bond between the carbonyl oxygen atom of Thr-1040 and the N atom of Gln-1353.