Figure 2.

IE86 increases hnRNP A2/B1 mRNA and protein expression levels. A. MouseIE86 levels were measured by Western blot. B. IE86 transgenic mice and wild-type mice were given the same feeding and environmental conditions. They were sacrificed at the tenth week to extract the brain tissue RNA and total protein; the relative mRNA expression of hnRNP A2/B1 was detected by qPCR. Results are representative of three independent experiments. C. Mouse hnRNP A2/B1 levels were measured by Western blotting. The blots are representative of three independent experiments. D. The IE86 levels in the U251 cells were measured by Western blot. E. U251 cells were transfected with peGFP-N3-IE86 as indicated for 24, 48, and 72 h, and the hnRNP A2/B1 levels were determined by qPCR. NC were cells not transfected with peGFP-N3-IE86. Results are representative of three independent experiments. F. Cell hnRNP A2/B1 protein levels were measured by Western blot. ** P < 0.01.