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. 2019 Jul 1;12(7):2775–2785.

Figure 3.

Figure 3

IE86 inhibits bcl-x splicing by promoting hnRNP A2/B1 expression. (A) Schematic splicing of Bcl-x pre-mRNA generating Bcl-xL and Bcl-xS mRNA via alternative 5’ splice site selection. (B) qPCR. U251 cells with stable hnRNP A2/B1 knockdown were grown in cell culture dishes and subjected to qPCR to determine hnRNP A2/B1 mRNA expression. The results are representative of three independent experiments, compared with the untransfected cell groups. (C) Western blot. The same cells were subjected to Western blot, compared with the untransfected cell groups. (D) After transfection with peGFP-N3-IE86 for 24 h, both U251 cells and cells transfected with peGFP-N3-IE86 were treated with sh1A2/B1. Afterwards, the Bcl-xL and Bcl-xS levels were determined by RT-PCR and (E) Western blot. *P < 0.05, **P < 0.01 and ***P < 0.001.