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. 2019 Jul 1;12(7):2526–2535.

Figure 3.

Figure 3

Sirt6 silence reduced 786-O renal cancer cell proliferation. A. Western blot tested the efficiency of small interfering RNA (siSirt6) and non-sense control (siCtl) in 786-O renal cancer cells. B. Images presented the cell density after siCtl or siSirt6 transfection for 48 h (scale bar: 200 μm). C. G1/S phase of cell cycle arrest were analyzed by using a flowcytometry after 786-O renal cancer cells were stained with PI. D. S phase cells were analyzed by using a flowcytometry after 786-O renal cancer cells were labeled by EDU for 2 hours. E. Colony number decreased after siSirt6 transfection. F. Ratios of apoptosis cells increased in 48-h siSirt6-transfected 786-O renal cancer cells analyzed by using a flowcytometry with apoptotic double staining. All data were presented as mean ± SEM. and analyzed by student’s t-test, *P < 0.05.