Hottip knockdown suppressed the fibrosis-related protein generation and extracellular matrix (ECM) accumulation in SV40-MES13 cells. A. The mRNA level of fibrosis-related protein including Col. I, Col. IV, FN and PAI-1 in kidney cortex of db/db and db/m mouse. B. The mRNA level of fibrosis-related protein including Col. I, Col. IV, FN and PAI-1 in mouse mesangial cells (MMCs, SV40-MES13) treated with high-glucose (25 mM) and low-glucose (5 mM) for 24 hr. C. Fibrosis-related protein expression in SV40-MES13 after Hottip knockdown and HG treatment. D. Grey scale analysis of fibrosis-related proteins. *P < 0.05. **P < 0.01.