Table 2. Steady-state kinetic parameters of various MICALs.
Protein | F-actin | k cat (s−1) | K m (µM) | k cat/K m (s−1 M −1) |
---|---|---|---|---|
hMICAL3FMOCH | — | 0.0006 | 266.9 | 2.0776 |
8 µM | 0.0169 | 543.8 | 30.9857 | |
hMICAL1FMOCH | — | 0.0023 | 1237 | 1.8238 |
8 µM | 0.0027 | 234.1 | 11.4267 | |
hMICAL3FMO | — | 0.0031 | 1264 | 2.4794 |
8 µM | 0.0024 | 817.2 | 2.8793 | |
hMICAL1FMO | — | 0.0005 | 573.7 | 0.8911 |
8 µM | 0.0006 | 752.6 | 0.8330 | |
hMICAL3FMOCHΔ213,530 | — | 0.0033 | 1438 | 2.3282 |
8 µM | 0.0025 | 1053 | 2.4169 |