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. 2019 Jun 1;12(6):2066–2074.

Figure 2.

Figure 2

Protective autophagy in MCF-7 cells and TAM-R cells. MCF-7 cells and TAM-R cells were plated in 60-mm dishes with 5% FBS IMEM, and treated with tamoxifen (10-7 and 10-6 M), and rapamycin (10 nM) in a culture medium for 24 hours when the cells were about 80% of confluence before immunoblotting. The protein bands were visualized and quantified using an Odyssey imaging scanner. A: The effects of tamoxifen on autophagy marker LC-3 in MCF-7 and TAM-R cells; B: The effects of tamoxifen on autophagy marker Beclin-1 in the MCF-7 and TAM-R cells; C: The effects of tamoxifen on autophagy marker p62 in the MCF-7 and TAM-R cells.