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. 2019 Aug 1;12(8):2898–2908.

Figure 2.

Figure 2

Elevated FGFR3 expression induces gene expression changes in spinal astrocytes. A. Immunofluorescence for FGFR3 (green) and GFAP (red) in the ipsilateral spinal dorsal horn of SNI and sham rat. B. Expression levels of FGFR3 in cultured spinal astrocytes which were infected with FGFR3 lentivirus or control lentivirus. Β-ACTIN was used as loading control. C, D. GFAP, and TNF-α mRNA levels were tested with q-RT-PCR in FGF2 (2 μM) treated for the periods indicated. Cultured spinal astrocytes were infected with FGFR3 lentivirus or controls. E. Effects of FGFR3 transfection on mRNA expression in cultured spinal astrocytes, including CCL-2/3, MCP-3, CXCL1, GDNF, and IL-1b. The astrocytes were treated with FGF2 (2 μM) for 48 hours. Results of three independent determinations are expressed as the mean ± SEM. *P<0.05.