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. 2019 Aug 1;12(8):2898–2908.

Figure 4.

Figure 4

FGFR3-TBX3 axis activation participates in TNF-α expression in cultured spinal astrocytes. A. After cultured spinal astrocytes were treated with PD173074 for 24 hr, TBX3 protein levels were measured with western blot assays. B. FGFR3 and TBX3 mRNA levels were tested with q-RT-PCR in PD173074 and FGF2 (2 μM) combined treated cultured spinal astrocytes for 24 hours. C. Cultured spinal astrocytes were treated with shFgfr3 or control lentivirus. Western blot assays were performed to measure the expression of FGFR3 and TBX3 after 24 hours treatment. D. Expression levels of GFAP and TNF-α was examined in shFgfr3 or control lentivirus treated spinal astrocytes by RT-PCR assays. Β-ACTIN was used as control.