Figure 2.

Effects of AB073614 expression on CRC cell proliferation. A. The relative levels of AB073614 were normalized to GAPDH in SW480 cell lines. SW480 was detected after the cells were transfected with si-AB073614, the AB073614 expression vector, or the control using real-time PCR. B. A CCK-8 assay was performed in SW480. Si-AB073614-transfected SW480 cells exhibited significantly lower growth rates than the control cells at day 3 after plating, and AB073614 vector transfected cells promoted cell growth. C, D. Cell proliferation in the si-AB073614, AB073614 expression vector or control transfected SW480 cells detected by an EdU staining assay.