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. 2019 Nov 1;12(11):4066–4076.

Figure 2.

Figure 2

Knock-down of hsa_circ_0057481 suppressed cell cycle progression and induced apoptosis in HEP-2 and TU212 cells. (A-C) FACS analysis to determine cell cycle distributions of HEP-2 and TU212 cells transfected with hsa_circ_0057481 specific siRNAs and control siRNA. (D-F) Apoptotic rates of HEP-2 and TU212 cells transfected with hsa_circ_0057481 specific siRNAs compared with control siRNA, which were evaluated by Annexin V-FITC/PI double staining. (G and H) Western blot analysis to determine the protein levels of Cyclin D1 and p27 in HEP-2 (G) and TU212 (H) cells transfected with hsa_circ_0057481 specific siRNAs and control siRNA. (I and J) Western blot analysis to determine the protein levels of BAX and BCL-2 in HEP-2 (I) and TU212 (J) cells transfected with hsa_circ_0057481 specific siRNAs and control siRNA. Three independent experiments were performed for each data point. *, P<0.05. **, P<0.01. ***, P<0.001.